5 Tips about flow cytometer You Can Use Today
5 Tips about flow cytometer You Can Use Today
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Information-enriched characterization of cell populations. Morphological information and facts from images can include for the richness of flow cytometry information. For example, the figure demonstrates an if not regular apoptosis assay working with Annexin V and PI, introducing mobile imaging to characterize cells in Just about every population to reveal morphologically distinctive attributes. These insights could not are received from multiplex staining alone.
Irrespective of whether you’re a beginner or an authority, investigate our methods to progress your study and experience in flow cytometry.
Mix acoustic focusing technological know-how with large-parameter analysis for flexible spectral unmixing and standard workflows.
Explore new insights into your experimental data by visualizing populations working with mixed fluorescence and morphology info. Our application interface wizards enable downsampling, concatenation (merged files) and dimensionality instruments which might be built to the software for an intuitive, streamlined knowledge analysis encounter.
"An image isn't really quite worth one thousand dots, but it surely truly really helps to see photographs. It is great to check out images that correlate with staining. As an example, AnnexinV-beneficial cells that are morphologically distinct than wholesome cells, or CD14+ monocytes which are larger sized and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it's illuminating to see how many doublets slip by way of standard singlet gates, and great to verify the standing of unanticipated double-constructive events in my analysis."
Pair your Attune flow cytometer by having an autosampler for greater workflow effectiveness, and increase robotic integration for optimum throughput.
Our experts will style and design panels in your case as many times you will need—totally free, no item buy required.
The Attune NxT cytometer is definitely the reputable workhorse instrument your lab can depend on for reliable and trusted functionality. The Attune CytPix design contains a significant-speed brightfield camera and AI-pushed impression analysis program, with current updates such as equipment Discovering applications and an intuitive person interface, enabling Increased information precision, accuracy, and a lot quicker insights into sample biology.
Users can check out occasion pictures in true-time to confirm sample top quality, enhance protocols, and report the morphology of every cell populace discovered for Assessment. Imaging capture also Gains from acoustic concentrating, which really helps to position the cells so that a sharp, centered impression is attained.
Seem waves help precise mobile alignment, resulting in Excellent facts quality and ~10X more quickly sample flow fees.
We Earlier demonstrated the strength of imaging Vehicle-T/Ramos cell interactions. Let’s evaluate just the population of finest curiosity, the double good activities, To find out more. We are able to now use prolonged morphological parameters (listed here, circularity and skewness of depth) to further more take a look at the attributes of these populations and refine gating on these gatherings, rising information robustness.
Designed-in dimensionality reduction applications elevated resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to verify expected populace resolution in lysed whole human blood. Consumer can look at occasions from Each and every cluster for visual confirmation of mobile phenotypes.
Flexible payment approach from Agilent Financial Alternatives allow you to get the important instrumentation you would like for here instant venture, or to expand your lab.
At the heart of each Attune Flow Cytometer lies our revolutionary acoustic focusing technologies, which makes use of seem waves to meticulously align cells inside the flow mobile. This ground breaking approach surpasses classic hydrodynamic strategies, causing more rapidly processing speeds, Remarkable information top quality, and greater sample throughput.
The Attune Xenith cytometer is usually a large-parameter spectral analyzer, giving the two spectral unmixing and traditional flow abilities for comprehensive and multipurpose cell Assessment.
Agilent Worth Promise ensures you a minimum of 10 years of instrument use with the date you purchase. If not, We'll credit score you with the residual price of the procedure towards an upgraded product.
In this article, we exhibit that by gating approaches working with picture-based mostly quantitative parameters can distinguish interacting cells from coincident occasions to far more precisely evaluate interacting cells.